Lab Notes & Best Practices

ELISA EXPERIMENT LOG
=====================

Date: [YYYY-MM-DD]
Operator: [Name]
Project: [Project Name]
Protocol Version: [v1.0]

PLATE LAYOUT (96-well):
- Samples: A1-A8, B1-B8, C1-C8
- Standards: D1-D8 (serial dilution)
- Positive Control: E1-E3
- Negative Control: E4-E6
- Blank Wells: E7-E8

REAGENT INFORMATION:
- Primary Antibody: [Catalog #] Lot: [####] Dilution: [1:1000]
- Secondary Antibody: [Catalog #] Lot: [####] Dilution: [1:5000]
- Substrate: [Type] Lot: [####]
- Buffer: [Type] pH: [7.4]

PROCEDURE NOTES:
1. Plate coating: Overnight at 4°C
2. Blocking: 1hr at RT with 5% BSA
3. Primary incubation: 2hr at RT
4. Secondary incubation: 1hr at RT
5. Development: 15min in dark

OBSERVATIONS:
- [Note any deviations from protocol]
- [Record any unusual observations]

RESULTS:
- Positive controls performed as expected: [Y/N]
- Negative controls within acceptable range: [Y/N]
- Standard curve R²: [value]
- Sample results: [attach data file]

NEXT STEPS:
- [Plan for follow-up experiments]
- [Any protocol modifications needed]

CELL VIABILITY ASSAY LOG
========================

Date: [YYYY-MM-DD]
Operator: [Name]
Cell Line: [Name/Passage #]
Plate Type: 96-well, tissue culture treated

EXPERIMENTAL DESIGN:
- Treatment Groups: Compounds A, B, C
- Concentrations: 10μM, 1μM, 0.1μM, 0.01μM
- Controls: Vehicle (DMSO), Untreated
- Replicates: n=6 per condition
- Total Wells Used: 72

PLATE MAP (96-well):
Row A-C: Compound A (triplicate concentrations)
Row D-F: Compound B (triplicate concentrations)  
Row G-H: Compound C, Controls, Blanks

CELL SEEDING:
- Density: 5,000 cells/well
- Volume: 100μL/well
- Media: DMEM + 10% FBS
- Seeding Date: [YYYY-MM-DD]

TREATMENT CONDITIONS:
- Treatment Start: 24hr post-seeding
- Treatment Duration: 48hr
- Final DMSO Concentration: <0.1%

ASSAY PROCEDURE:
- MTT addition: 20μL/well (5mg/mL)
- Incubation: 4hr at 37°C, 5% CO₂
- Solubilization: 100μL DMSO
- Reading: 570nm absorbance

QUALITY CONTROLS:
- Untreated Control Viability: [%]
- Vehicle Control Viability: [%]
- Positive Control (if used): [result]

RESULTS SUMMARY:
- IC₅₀ Values: [Compound A: X μM, Compound B: Y μM, Compound C: Z μM]
- Statistical Analysis: [Method used]
- Data File: [filename.xlsx]

qPCR EXPERIMENT LOG
===================

Date: [YYYY-MM-DD]
Operator: [Name]
Instrument: [Model]
Protocol: [Name v1.0]

SAMPLE INFORMATION:
- Sample Type: [cDNA/gDNA/RNA]
- Extraction Date: [YYYY-MM-DD]
- Storage: -80°C
- Sample IDs: [S1, S2, S3...]

PRIMER INFORMATION:
Target Gene 1: [Gene Name]
- Forward: [Sequence] Tm: [°C]
- Reverse: [Sequence] Tm: [°C]
- Product Size: [bp]

Reference Gene: [Gene Name]
- Forward: [Sequence] Tm: [°C]
- Reverse: [Sequence] Tm: [°C]
- Product Size: [bp]

REACTION SETUP (per well):
- Master Mix: [Type] 10μL
- Primer Mix (F+R): 1μL each
- Template: 1μL
- Water: 7μL
- Total Volume: 20μL

PLATE LAYOUT:
- Samples: A1-H6 (target gene)
- Samples: A7-H12 (reference gene)
- No Template Controls: G7, H7, G12, H12
- Standards: (if applicable)

CYCLING CONDITIONS:
- Initial Denaturation: 95°C, 10min
- Cycling: 40 cycles
  - Denaturation: 95°C, 15sec
  - Annealing: [°C], 30sec
  - Extension: 72°C, 30sec
- Melt Curve: 65-95°C

QUALITY CONTROLS:
- NTC Ct Values: [Should be >35 or undetermined]
- Reference Gene Stability: [acceptable range]
- Amplification Efficiency: [90-110%]
- R² Value: [>0.99]

RESULTS:
- Average Ct Values: [table/file reference]
- ΔΔCt Analysis: [method used]
- Fold Change: [results summary]
- Statistical Analysis: [method, p-values]

DATA FILES:
- Raw Data: [filename.eds/.xml]
- Analysis: [filename.xlsx]
- Plots: [filename.pdf]